Identification of blood group antigens and minor cell populations by the fluorescent antibody method.

“SHE APPLICATION of the fluorescent antil)odiv technic for the identification of blood group antigens in crythrocvtcs offers a method of invcstigation of a variety of theoretical anci practical problems in imrnuunohematobogv and genetics. Iii 1958, studies from this laboratory were rcportcdim in which sj)ccific fluorescence of group A and group B crvthrocvtcs was achieved with htuuiiaii anti-A and anti-B sera conjugated with fluorescein isocvanatc. Since then, we have cxtcncicdl these observations audi succccdedl in labeling Rh and other i)lOOdi group factors with fluorescent antisera of appropriate s1)ecificitV. It s’as imniediiatcly 11)1)ctrciit that the niethodi might bend itself to the detection an(i